Substrate Setup for Live Plants By Shirlie Sharpe Shirlie Sharpe Shirlie Sharpe is an aquatic expert and writer with over three decades of experience keeping and raising ornamental fish. She has consulted with The Minnesota Zoo and the National Aquarium. Shirlie has also authored a book on setting up aquariums. Learn more about The Spruce Pets' Editorial Process Updated on 12/30/25 Reviewed by Nick Saint-Erne Reviewed by Nick Saint-Erne Dr. Nick Saint-Erne, DVM, is a highly accomplished veterinarian and writer who has treated zoo animals and exotic pets for more than 35 years. He has worked with the Centers for Disease Control and Prevention to improve pet store animal care among other endeavors. Dr. Saint-Erne is part of The Spruce Pets' veterinary review board. Learn more about The Spruce Pets' Veterinary Review Board Aquarium Substrate. Credit: Haley Hughes Key Takeaways Choose a substrate that maintains stable water conditions, avoiding materials that alter pH or hardness significantly.Layer the substrate with fertilizer at the bottom and finer granules on top to support plant growth.Match substrate depth to plant root types, with deep-rooted plants requiring at least 6 cm of substrate. The substrate is the material on the floor of the tank where the aquarium plants will root. The most important factor is that the substrate should be a material that does not adversely affect the water conditions by raising or lowering pH and water hardness. You want a happy medium. Crushed coral or shells and certain kinds of limestone gravel will create a high pH and high carbonate hardness, which is not good for your aquarium plants. On the other hand, low pH and very soft water can cause root-rot, which in turn will lead to algae growth. A pH of 6.8 to 7.2 is pretty much ideal, depending on the type of aquarium plants you select. Stick to natural substrates, avoid synthetic materials, such as gravel that is coated with epoxy or glass beads and colored ceramics. Substrate Size Substrate material should be between 3 and 8 mm thick. Large granules will block root growth and smaller ones can actually crush the roots. It is recommended when starting the tank that you add the substrate in phases. For example, mix the first layer of solid fertilizer, (remember - less is more) and place that in the tank. Then add as much as two additional layers of gravel with the finest granules on the top layer. Note that the additional layers do not contain fertilizer. Remember to slowly pour the water into a bowl or plate on the bottom when filling with water in order to avoid churning the fertilizer up. Substrate Depth You also have to provide substrate at the proper depth for your aquarium plants. There are four groups of aquatic plants that can be classified by root type. Plants that don't need sand but attach their roots to rocks or wood such as Anubias, Microsorium, and Bolbitis.Plants with large rootstocks like Aponogeton and Nymphaea.Plants with long stems like Hygrophila and Rotala that have shallow roots.Plants like Cryptocoryne and Echinodorus that are deep-rooted. To give you an idea of the depth of the substrate, you must consider the type of aquarium plants you are going to use. The deep-rooted ones will require the most depth. If they are planted in the substrate that is not deep enough, the roots will become entangled and the aquarium plants will suffer from a lack of nutrients. The deep-rooted plants need at least a 6 cm deep substrate (2 to 3 inches). Substrate Material Substrate materials for planted aquariums are abundant now, with two of my favorites being CaribSea Eco Complete, and SeaChem Fluorite. Growth is lush and these substrates appear to be complete in terms of what the plants need. It does seem that there is a lag period for the Eco Complete in terms of plant growth of about 2 to 3 weeks. In other words, the plants do not take off for that period of time. If you are interested in doing a soil-based substrate, get Ecology Of The Planted Aquarium by Diana Walstad; it is a complete book on how to, plus some scientific study on aquatic plants. Explore more: Fish & Aquariums Aquarium Care & Maintenance